an in vitro method for assessing the efficacy of antivenom against hemiscorpius lepturus venom
نویسندگان
چکیده
background hemolyis of red blood cells is a serious toxic effect commonly found among patients envenomed by hemiscorpius lepturus scorpion. objectives the aim of the present study was to evlaute the efficay of the avaible polyvalent antivneom in preventing this phenomena. materials and methods using a red blood cell fragility test, the anti-hemolytic effectiveness of a new antivenom serum against hemiscorpius lepturus venom was investigated. hemolysis was measured using spectrophotometry. results addition of venom (2, 10, 20, and 40 µg/ml) to 0.5 ml of 5% washed red blood cell suspension produced concentration-dependent hemolysis. both the pre-incubation of red blood cell suspensions with various concentrations of antivenom (4%, 10%, and 20% v/v) and the co-administration of antivenom with 20 µg/ml venom resulted in concentration-dependent protection against hemolysis. both the methods resulted in protection against hemolysis at the antivenom concentration of 20% (v/v). however, the inhibition of hemolysis after 24 h was found to be greater for red blood cell suspensions preincubated with antivenom (75% inhibition) than for red blood cell suspensions that were co-administered with antivenom and venom (50% inhibition). conclusions the results suggest that the antivenom against h. lepturus venom is useful in inhibiting hemolysis produced by the venom, but the duration of protection is relatively short and appropriate measures need to be taken, depending on the patients’ clinical progress, to re-administer the antivenom at intervals less than 8 h. this proposed treatment method merits further clinical assessment.
منابع مشابه
An In Vitro Method for Assessing the Efficacy of Antivenom Against Hemiscorpius lepturus Venom
BACKGROUND Hemolyis of red blood cells is a serious toxic effect commonly found among patients envenomed by Hemiscorpius lepturus scorpion. OBJECTIVES The aim of the present study was to evlaute the efficay of the avaible polyvalent antivneom in preventing this phenomena. MATERIALS AND METHODS Using a red blood cell fragility test, the anti-hemolytic effectiveness of a new antivenom serum a...
متن کاملIn vivo pharmacological study on the effectiveness of available polyclonal antivenom against Hemiscorpius lepturus venom
The available Razi Institute antivenom is still, empirically, used by intramuscular (IM) administration for the treatment of scorpion stings in humans by six medically dangerous species including Hemiscorpius lepturus (H. lepturus). The aim of this study was to assess the neutralizing ability and effectiveness of the antivenom in inhibiting hemoglobinuria, biochemical changes, increased microal...
متن کاملin vitro pharmacological study upon the effectiveness of available antivenom against hemiscorpius lepturus venom
متن کامل
The pharmacokinetics of Hemiscorpius lepturus scorpion venom and Razi antivenom following intramuscular administration in rat
Hemiscorpius lepturus (H. lepturus) is one of the most dangerous scorpions in Iran. Intramuscular administration (IM) of available Razi antivenom to H. lepturus venom is used by many of Iranian clinicians. The purpose of the current study was to investigate the efficiency of IM route for treatment of envenomed patients by H. lepturus. We compared the pharmacokinetics parameters of venom and ant...
متن کاملA biodistribution study of Hemiscorpius lepturus scorpion venom and available polyclonal antivenom in rats
The purpose of the present study was to investigate the biodistribution profile of the venom of Hemiscorpius lepturus, the most dangerous scorpion in Iran. Blood and tissue samples were taken at various predetermined intervals during a 400-minute period for the venom and a 360-minute period for the antivenom in rats. The radio-iodination was carried out using the chloramine-T method. The result...
متن کاملToxic Effects of Scorpion, Hemiscorpius Lepturus (hemiscorpiidae) Venom on Mice
Scorpion stings are common in Iran, particularly in the southern and southwest regions of the country, and they pose a serious health problem. Hemiscorpius lepturus is the most venomous scorpion in the region. The present study was conducted to investigate the in vivo effects of scorpion venom in experimental mice. Haematological parameters (white and red blood cells numbers and haematocrit lev...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
jundishapur journal of natural pharmaceutical productsجلد ۷، شماره ۱، صفحات ۳۵-۳۸
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023